The major concern of the study is to determine the level of preparedness and level of performance in observation and participation of male and female student teachers. The researcher used the causal-comparative method of research. The respondents of the study were the 70 student teachers of the Don Honorio Ventura Technological State University (DHVTSU) who undergone observation and participation practice during the first semester of school year 2014-2015. The teachers were found to be much prepared and exhibited superior performance in observation and participation. The findings revealed that the male and female student teachers have different levels of preparedness in teaching. Based on the positive p- values, it can be deduced further that the male respondents are more prepared than the female respondents. Perhaps the male student teachers are more knowledgeable of their topics, they can adjust their teaching methodologies considering the needs of the pupils/students, they are more punctual and they even use specific criteria in evaluating the performance their pupils/students.